Purification, some properties and the specific biological activity of cytochromes c4 and c5 from Azotobacter vinelandii.
نویسنده
چکیده
We should like to thank Mr D. M. Wallace for his help in diagnosis and in arranging for the collection of specimens. This research was supported by grants to the Royal Cancer Hospital and Chester Beatty Research Institute from the British Empire Cancer Campaign, the Jane Coffin Childs Memorial Fund for Medical Research, the Anna Fuller Fund and the National Cancer Institute of the National Institutes of Health, U.S. Public Health Service.
منابع مشابه
Cytochrome c oxidase from Azotobacter vinelandii.
Although the terminal respiratory chain of Azotobacter has been the subject of several recent studies, the nature of the cytochrome oxidase portion is still somewhat obscure. Repaske (1) partially purified and characterized a soluble succinic dehydrogenase and succinoxidase system from Azotobacter z&elan&i. Wilson and Wilson (2) studied the soluble succinoxidase in Repaske’s preparation and dem...
متن کاملA comparison of the physical and chemical properties of four cytochromes c from Azotobacter vinelandii.
1. A modified method for the separation and purification of four cytochromes c from Azotobacter vinelandii is described. Two new cytochromes c have been purified and are designated cytochromes c(551) and c(555). 2. Additional evidence is presented to establish the dihaem nature of cytochrome c(4). Ultracentrifugation data indicated similar molecular weights for the native and the denatured prot...
متن کاملThe amino acid sequence of the dihaem cytochrome c4 from the bacterium Azotobacter vinelandii.
An amino acid sequence is proposed for the cytochrome c4 from the bacterium Azotobacter vinelandii strain OP. It is a single polypeptide chain of 190 residues, with two sets of haem-attachment cysteine residues at positions 14/17 and 119/122. Proteins with similar sequences are also present in denitrifying pseudomonads. There is similarity in sequence between the two halves of the cytochrome c4...
متن کاملFree and membrane - bound forms of bacterial cytochrome c 4 Graham
Cytochrome c4 was isolated from cells of Pseudomonas aeruginosa, Pseudomonas stutzeri and Azotobacter vinelandii. The dihaem nature, Mr of approx. 20000 and ferrohaem spectra in the region of the aand ,peaks define this family of cytochromes c. The behaviour of the holocytochromes in SDS was atypical, but removal of the haem groups resulted in a normal migration. In all three organisms most of ...
متن کاملNMR assignments of 1H, 13C and 15N resonances of the C-terminal subunit from Azotobacter vinelandii mannuronan C5-epimerase 6 (AlgE6R3)
The 19.9 kDa C-terminal module (R3) from Azotobacter vinelandii mannronan C5-epimerase AlgE6 has been (13)C, (15)N isotopically labelled and recombinantly expressed. We report here the (1)H, (13)C, (15)N resonance assignment of AlgE6R3.
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ورودعنوان ژورنال:
- The Biochemical journal
دوره 64 3 شماره
صفحات -
تاریخ انتشار 1956